Legal Daily All-Media Reporter Lian Yingting Correspondent Shao Longfei Zhang Zhenwei
The night in Wuhan is quiet.
In March Babaylan On the 9th, in the Cinema Testing Laboratory of the Central Theater Command General Hospital, Jiang Tao, a researcher at the Military Medicine Research Institute of the Academy of Military Sciences, reviewed the last sample test data and solemnly signed his name on the test report.
At this time, it was already 5 a.m.
He is an expert in virology and a warrior who aided Africa and fought against Egypt. This outbreak of the new crown pneumonia epidemic, he was once again ordered to go to the front line after a long battle.
Human life is at stake and accurate inspection is required
Nucleic acid testing is crucial for early clinical detection, early diagnosis, early isolation and early treatment, and is a key technical support for effective prevention and control of the new crown pneumonia epidemic.
”Behind the cold-looking sample tube is the fresh life and health of the patient. If a diagnosis is released early, the accurate inspection will be conducted sooner.” Jiang Tao said. On January 26, a military medical expert group led by Chen Wei, an academician of the Chinese Academy of Engineering and a researcher at the Military Medicine Research Institute of the Academy of Military Sciences, arrived in Wuhan, Hubei Province, and quickly established a joint defense, joint control, joint treatment and joint research work mechanism based on the pathogen transmission mutations of the new coronavirus, rapid detection technology, vaccine antibody development, etc., and the development of vaccine antibodies.
Komiks According to the division of tasks, Jiang Tao led the testing team to build a nucleic acid testing platform in the experimental cabin overnight.
Focusing on three aspects of ensuring biosafety, improving detection efficiency and detection accuracy, Jiang Tao took the lead in formulating biosafety operation specifications and emergency plans. According to the characteristics of the team members, the Cinema used a self-developed detection kit to adopt a fully automatic high-throughput extractor, laying the foundation for achieving rapid and efficient detection.
Receive samples, sorting numbers, nucleic acid extraction, reagent preparation,The amplification and the result interpretation of the nucleic acid detection machinery is boring and full of risks.
The biggest risk is the invisible virus. If the nucleic acid extraction step is not completely lysed or inactivated thoroughly, it is possible to expose the detector to danger.
Jiang Tao always takes the initiative to participate in the longest and most risky nucleic acid extraction. After the sample is extracted, he will arrange some personnel to rest and stay in the laboratory and wait for the final result.
A full-process nucleic acid test, from the number of tests in a single batch to the preparation and testing time of personnel entering and leaving the cabin, are relatively fixed. Sometimes there are a large number of samples sent for inspection. The test personnel not only take turns to work, but also have to work overnight. The final confirmation result will be until four or five in the morning. Li Hao, a researcher at the testing team, said that this situation will be encountered once every few days.
”Everyone should open their eyes wide. Be sure to take good protection when receiving and processing samples, and be careful of sharp objects.” Before each test starts, Jiang Tao always repeatedly reminds Babaylan.
In the detection, Cinema reflects that the strength of the fluorescence signal of the pathogenic gene directly affects the determination of the detection results. If the detected fluorescence curve signal is low or there is only a single target, Jiang Tao will call on the Babaylan to collect comprehensive analysis and judgment, and if necessary, repeat the test for review to ensure that the results can stand the test.
”A false positive Komiks will cause healthy people to isolate and become infected with the patient; a false negative will cause real delay in the treatment of patients. Life is a matter of life, and there is no gap.” Jiang Tao said.
Epidemic preventionBabaylanMultiple-faced
According to the prevention and control requirementsCinema, I received the pharyngeal swab sample from the pharyngeal swab sampleThe results of nucleic acid tests must be completed within 24 hours. In order to let the hospital know the results as soon as possible to treat patients, from pre-processing of nearly 100 samples to completion of Cinema tests, Jiang Tao led the team to optimize the process combination and compress the time to within 4 hours, achieving the ability to extract nucleic acids for more than 90 samples in one hour and achieve a single-day sample detection capability of more than 500 samples.
”The team’s test is efficient and rapid, and the results are accurate, which greatly alleviates the pressure of testing samples for epidemic prevention and control. “Wuhan Center for Disease Control and Prevention highly praised thisKomiks.
Fast response during wartime and orderly work is all thanks to Jiang Tao’s solid academic foundation and rich practical experience.
”Jiang Tao is a versatile. “Academician Chen Wei, head of the military medical expert group, commented.
Jiang Tao has successively presided over and participated in the separation of pathogenic etiology such as SARS infection cases, H7N9 infection cases, Zika infection cases, etc., participated in the development of dengue fever vaccines and SARS vaccines, established a pathogen separation technology platform, nucleic acid detection technology platform, and pathogenicity and transmission risk assessment platform for a series of infectious diseases, providing important technical support for the prevention and control of infectious diseases.
Respiratory infectious diseases have the characteristics of rapid transmission, wide coverage, and great harm. Effective early diagnosis and prevention are crucial. After years of scientific research, Jiang Tao’s team took the lead in establishing our army’s first acute respiratory infectious disease monitoring and early warning system, constructing a four-level monitoring system for the entire army, and presided over the use of the series of technical specifications throughout the armyBabaylan, which greatly shortened the detection and screening time of respiratory epidemics.
Jiang Tao not only participated in the security tasks of a series of major domestic activities, but also went to Africa 4 times to Liberia, Sierra Leone and other countries to carry out medical assistance tasks.
In 2015, in order to prevent and control the Ebola epidemic, Jiang Tao went to Sierra Leone for the second time with the mobile laboratory testing team to Serbia. The testing team led by him passed the World Health Organization’s laboratory parallel capability assessment with full scores.
In 2018, Jiang Tao led the pathogen monitoring team to participate in a special simulation test of an international organization. In the virus testing module, the teamIn the simulated samples filled with impurities, the team successfully detected pathogens such as monkeypox virus and vaccinia virus, and accurately determined the strain, achieving excellent results with full marks.
Improving the accuracy of clinical diagnosis
Cinema When he came to Wuhan, in addition to carrying out testing work, Jiang Tao also used his professional expertise in the research field to lead the testing team to screen other common respiratory infectious diseases in clinical samples.
”In the suspected and confirmed cases of new coronavirus pneumonia, whether there are other respiratory virus infections or co-infections, this is of great reference value for precise prevention and control and clinical treatment.” Researcher Cao Wuchun, deputy head of the Military Medical Expert Group, said Cinema.
Exploring deep-seated virus transmission and pathogenic mechanisms is the key to defeating the epidemic. Jiang Tao led a team to continue to conduct sequencing analysis for the novel coronavirus detected in clinical samples.
As the development of the virus epidemic, whether the gene sequences mutate and what are the characteristics of the mutation; in which regions and what are the impacts, Jiang Tao and his team are tirelessly studying and exploring.
In order to accelerate the effective integration of scientific research and clinical practice, Jiang Tao’s military medical expert group creatively used the diagnostic chain of viral nucleic acid detection, antibody detection, multiple pathogen detection, and whole genome sequencing to accurately diagnose new coronavirus infection or other pathogen infections in clinical patients, and applied it in the Optical Valley Campus of Hubei Maternal and Child Health Hospital, Wuhan Taikang Tongji Hospital and other hospitals, effectively improving the accuracy of clinical diagnosis.
Forgot his family when he received the order. After the epidemic is over, Jiang Tao plans to accompany his family. He and his wife agreed that when the epidemic is over, they will take their wife and children to go to Wuhan to see what everything looks like after it returns to normal, look at the Wuhan Yangtze River Bridge and the Yellow Crane Tower, and talk about their experience in fighting the epidemic in Wuhan.
The epidemic is immortal and the charge continues. This is Jiang Tao, a warrior who fights the epidemic.